|
Overview :
The net negative charge associated with a serum protein can
be estimated based on its concentration and physiochemical features.
net negative charge in mEq/L =
= (serum concentration in g/L) * (buffer value for the
protein) * ((pH) – (isoelectric point for the protein))
where:
• The buffer value for most serum proteins is 0.1 mmol per g
per unit pH
Interpretation:
• A total serum
protein of 7 g/dL at body temperature and pH of 7.4 would have a net negative
charge of 12-16 mEq/L.
|
Serum Protein
|
Isoelectric Point
|
|
albumin
|
4.9
|
|
alpha-2-macroglobulin
|
5.4
|
|
transferrin
|
5.8
|
|
beta-1-lipoprotein
|
5.4
|
|
IgG
|
7.3
|
|
fibrinogen
|
5.4
|
from Geigy Scientific Tables, Seventh Edition, page 580.
|