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Overview :
The net negative charge associated with a serum protein can
be estimated based on its concentration and physiochemical features.
net negative charge in mEq/L =
= (serum concentration in g/L) * (buffer value for the
protein) * ((pH) – (isoelectric point for the protein))
where:
• The buffer value for most serum proteins is 0.1 mmol per g
per unit pH
Interpretation:
• A total
serum protein of 7 g/dL at body temperature and pH of 7.4 would have a net
negative charge of 12-16 mEq/L.
|
Serum
Protein |
Isoelectric
Point |
|
albumin |
4.9 |
|
alpha-2-macroglobulin |
5.4 |
|
transferrin |
5.8 |
|
beta-1-lipoprotein |
5.4 |
|
IgG |
7.3 |
|
fibrinogen |
5.4 |
from Geigy Scientific Tables, Seventh Edition, page 580.
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