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 Release 21.0, Jan 2008
 
Chapter : ch11. Hepatobiliary & Pancreas Section : Diagnosis and Prognosis of Pancreatitis
  Analysis of Amylase and Lipase Results in the Diagnosis of Pancreatitis

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Overview :

The diagnosis of acute pancreatitis can be difficult to make, especially when mild, late in its course or when multiple other disorders are present.

 

Testing: Amylase and lipase should be tested simultaneously. Results for both may be expressed as multiples of the upper limit of normal.

 

lipase as multiple of upper limit of normal =

= (serum lipase in patient) / (upper limit of reference range for lipase)

 

amylase as multiple of upper limit of normal =

= (serum amylase in patient) / (upper limit of reference range for amylase)

 

Interpretation

 

Acute pancreatitis is highly likely if:

(1) The serum lipase is > 5 times the upper limit of normal.

(2)  Increases then decreases in serum amylase and lipase are in concert.

(3) Serum lipase values show significant change over time.

 

Elevations in amylase and lipase less than 3 times the upper limit of normal can be seen in conditions other than pancreatitis, including drug effect or acute abdomen with bowel infarction or perforation.

 

Very high elevations in amylase (greater than 25 times the upper limit of normal) can be seen in metastatic cancers.

 

An increase in ALT to 3 or more times normal suggests gallstone-induced pancreatitis.

 

Limitations:

• Amylase by itself is relatively insensitive for making the diagnosis of pancreatitis. It may be normal at presentation and tends to fall to normal more rapidly than lipase, so that in late pancreatitis only the lipase is elevated.

• The magnitude of increase in amylase levels may not correlate with severity of underlying disease.

• Amylase of salivary gland origin needs to be distinguished from amylase of pancreatic origin.

• Many drugs can cause mild increases in both amylase and lipase without evidence of acute pancreatitis.

 

  References:

Corsetti JP, Cox C, et al. Combined serum amylase and lipase determinations for diagnosis of suspected acute pancreatitis. Clin Chem. 1993; 39: 2495-2499.

Jacobs D, Demott W, et al. Laboratory Test Handbook, 4th edition. Lexi-Comp Inc. Hudson, Ohio. 1996. page 158.

Kazmierczak SC, Catrou PG, Van Lente F. Enzymatic markers of gallstone-induced pancreatitis identified by ROC curve analysis, discriminant analysis, logistic regression, likelihood ratios and information theory. Clin Chem. 1995; 41: 523-531.

Ladenson JH, Scott MG. et al. The clinical chemistry laboratory and acute pancreatitis. Clin Chem. 1993; 39: 234-243.

Orebaugh SL. Normal amylase levels in the presentation of acute pancreatitis. Am J Emerg Med. 1994; 12: 21-24.

Steinberg W, Tenner S. Acute pancreatitis. N Engl J Med. 1994; 330: 1198-1210.

Ventrucci M. Update on laboratory diagnosis and prognosis of acute pancreatitis. Dig Dis. 1993; 11: 189-196.

Wallach J. Interpretation of Laboratory Tests, 6th edition. Little Brown and Company. 1996. page 231.

 

 

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